Since there is a wide selection of gels, please follow the 7 steps below
to create a catalog number for the Gels you need.
Call us at 1-800-648-1772 for assistance.
Here is a sample part number: 12D15HMC10P Step 1.Select Acrylamide
Concentration: We will pour almost any single
concentration or gradient concentration
gel.
i.e. 12 = 12% single concentration (Specify any single concentration).
420 = 4%-20% gradient (Pick any gradient you need).
Not sure what percent acrylamide gel to choose?
Step 2.Select the Gel
Type: (learn more about buffer
systems) D = Tris-Glycine Gels with SDS, (Gel buffer is: 0.375M Tris-HCl, pH 8.8,
.1% SDS)
W = Tris-Glycine Gels without SDS (Gel buffer is: 0.375M Tris-HCl, pH 8.8)
N = Tris-Borate-EDTA (TBE), (Gel Buffer is: 0.090M Tris, 0.080M Boric Acid, pH
8.3, 0.0026M EDTA). TBE Gels do not come with a 4% stacking gel. U = Urea/TBE Gels ( Same as N Gels, with 7 molar Urea added). TBE/Urea
Gels do not come with a 4% stacking gel.
T = Tris-Tricine Gels (ideal for low molecular weight 2 - 60 kilo-Daltons), (Gel
Buffer is: 1.0M Tris-HCl, pH 8.45, 0.1% SDS).
VOTE:
We are considering making mini gels in a 1.0 mm
thickness. Please send us an email if you prefer 1.00 mm thick mini gels.
Service@precastgels.com
Simply type 1 mm Gels in the subject line or leave more
information and we can contact you when they are available. Thank you!
Step 4.Select your
Running Apparatus:
MINI GELS: HM = Amersham (Hoefer) Mighty Small, SE250, SE260,
SE280,
Thermo EC120, Fisher Biotech FB VE10-1, or Owl Penguin P8DS
NM = (Universal Cassette) Novex/Invitrogen XCell I, II, and Surelock, BioRad Mini Protean 3
CBS models MGV-302 and 402, Sigma Aldrich E5889 Vertical Unit.
BM = Bio-Rad Mini Protean II (but not Bio-Rad Mini Protean 3, see NM) SM = Owl/ISS/Enprotech Min Apparatus or Hoefer SE200 use glass cassettes. These
glass mini cassettes also fit into the same apparatus as "HM" and "BM".
These mini cassettes 8cm tall x 10cm wide are made with low fluorescence glass.
LARGE SIZE GELS
HL = Amersham (Hoefer) SE400, SE600, SE650, SE700
(Standard gel width is 14 cm). BL = Bio-Rad Protean II and IIxi, Owl P10DS-1,-2,-3 (Owl requires sponge tips,
ST-10). (Standard Gel width is 16 cm. An 18 cm
wide gel can be obtained by requesting 1.0 cm wide spacers. Cassettes
dimensions: front plate 16cm tall x 20 cm wide and back plate 18.3 cm tall by 20
cm wide.)
Step 5.Identify the Number of Sample Wells you want:
C10 = 10 wells C15 = 15 wells C1 = 1 well. The resolving gel is poured 1cm from the top (.5cm from the top for mini gels)
to create a flat top for prep and 2D work
(see 2D gels) . The
C1 designation does not include a stacking gel. If a stacking gel is
desired, you must specify it. C2 = Same as C1 except there is also a reference well at one end.
Available on "HL", "BL","BXL", "SM", and "EL"
for all gel thicknesses (.75mm, 1.00
mm and 1.50 mm thickness).
Effective October 10, 2008 the price for a sample well is $2.00 per gel for gels
in glass cassettes.
Available on mini gels with designation "HM", "BM",
"NM" in 1.5mm
gel thickness only size Gels (no extra charge).
The C2 designation does not include a stacking gel. If a stacking gel is
desired, you must specify it.
C0 = no sample wells (mini gels poured 1.5 cm from top, large gels poured 3.5 cm
from top)
Step 6.Select a Plastic or Glass Cassette for your
gels:
P = Plastic Cassette (available for 'HM', 'BM', 'NM' styles and running one gel
in "SM" style) G = Glass Cassette (all large gels use glass cassettes. Use glass to run 2 gels
in "SM")
GLF=low
fluorescence glass (available for "EL", "HL", "BL" and "BXL"). "EL" are made
with Low fluorescence glass. "HL" and "BL" can be made with regular glass
"G" or low fluorescence glass "GLF". Our Mini Gels
"SM" style are made with low fluorescence glass also.
Does Your Part Number Look Similar To This Sample? 420D75HMC10P Mini gels are sold 10 per box or 2 per box (glass
cassettes 8 per box or 2 per box),
Large size gels are sold 6 per box or 2 per box.
You can order more than 2, 6, 8 or 10 gels quantities. You may order gels by phone, fax, or e-mail
or
Order Online.
To
Choose the Gels you need follow these 4 Steps:
See images of actual proteins run on gels.
Develop a part number by choosing the options
available such as: gel concentration, gel buffer, thickness, cassette
size, sample wells, etc.
See prices of all gel types and Sizes.
How to order information: Fax number, Phone number or
online ordering.
DOWNLOADS
Information on power settings, sample well volumes, etc.