Please follow the 7 steps below to create a Gel
Call us at 1-800-648-1772 for assistance.
Here is a sample part
Enter: 10,12, or any single or gradient
concentration, example, 420, 1020 etc.
We will pour almost any
single concentration or gradient concentration gel.
= 12% single concentration (Specify
any single concentration). Single concentration gels
with sample wells are provided with a 4% stacking gel layered on top of the
resolving gel. 420
= 4%-20% gradient (Pick any gradient
what percent acrylamide gel to choose?
Enter: D,W,N, U,T,A or B.
(more about buffer systems) D= Tris-Glycine Gels with SDS, (Gel buffer is: 0.375M
Tris-HCl, pH 8.8, .1% SDS) W
= Tris-Glycine Gels without SDS (Gel buffer is: 0.375M
Tris-HCl, pH 8.8) N= Tris-Borate-EDTA (TBE), (Gel Buffer is: 0.090M
Tris, 0.080M Boric Acid, pH 8.3, 0.0026M EDTA). TBE Gels
do not come with a 4% stacking gel. U= Urea/TBE Gels ( Same as N Gels, with 7 molar Urea
added). TBE/Urea Gels do not come with a 4% stacking gel.
= Tris-Tricine Gels. Ideal for small proteins from 2.3 to
100 kD. (Gel Buffer is: 1.0M Tris-HCl, pH 8.45, 0.1% SDS).
= Tris Acetate Gels. Ideal for large size
proteins up to 500 kD.
B= Blue Native Gels. New!
Ideal for study of protein-protein interactions and very
hydrophobic proteins, such as membrane proteins, their
complexes and super complexes. (as described by Schagger and
von Jagow, Analytical Biochemistry (1991) 199,
Enter: 15,10 or 75
Select Gel Thickness: 15=
1.50 mm thick 10 =
1.00 mm thick 75 =
0.75 mm thick
Enter: HL, BL or BXL
Select your Running Apparatus: HL =
Fits into GE Healthcare (Manufactured by
Hoefer, Inc.) SE400, SE600, Ruby SE600, SE650, SE660, SE700.
in regular and low fluorescence glass.
Fits into Bio-Rad Protean II, II xi and XL, Protean II XL
Multicell, Owl P10DS-1,-2,-3 (Owl requires sponge tips,
ST-10). Cassette dimensions:
front plate 16cm tall x 20 cm wide and back plate 18.3 cm
tall by 20 cm wide. Available in regular and low
fluorescence glass plates.
BXL = (longer cassette) Fits into Bio-Rad Protean
II xi and XL, Protean II XL Multicell, and Protean Plus
Dodeca Cell (for 2D electrophoresis only).
Cassette dimensions: Front plate 20cm
tall x 20 cm wide, back plate 22.3 cm tall x 20 cm wide.
Available in regular and low fluorescence glass.
= Same as C1 except there is also a sample well (for loading
a protein standard) at one end. The C2
designation does not include a stacking gel.
The Gels are made with
2 cm wide spacers, which create an 16 cm wide gel minus 1 cm
for the sample well. A 14.5 cm long IPG strip would fit.