Please follow the 7 steps below
to create a catalog number for the Gels you need.
Call us at 1-800-648-1772 for assistance.
Here is a sample part number: 12D15HMC10P
Step 1.
Select Acrylamide
Concentration: We will pour almost any single
concentration or gradient concentration
gel. 12 = 12% Specify any single concentration.
Single concentration gels are provided with a 4% stacking gel layered on top of
the resolving gel, except for gels with catalog designation C1G or C2G,
which have a flat top. 420
= 4%-20% gradient (Pick any gradient you need).
Not sure what percent acrylamide gel to choose?
Click here?
Step 2.
Select the Gel
Type: (learn more about buffer
systems) D = Tris-Glycine Gels with SDS, (Gel buffer is: 0.375M Tris-HCl, pH 8.8,
.1% SDS) W = Tris-Glycine Gels without SDS (Gel buffer is: 0.375M Tris-HCl, pH 8.8) N = Tris-Borate-EDTA (TBE), (Gel Buffer is: 0.090M Tris, 0.080M Boric Acid, pH
8.3, 0.0026M EDTA). TBE Gels typially not supplied with a 4% stacking gel. U = Urea/TBE Gels ( Same as N Gels, with 7 molar Urea added). TBE/Urea
Gels do not come with a 4% stacking gel.
T = Tris-Tricine Gels. Ideal
for small proteins from 2.3 to 100 kD. (Gel
Buffer is: 1.0M Tris-HCl, pH 8.45, 0.1% SDS).
A= Tris-Acetate Gels. Best for High Molecular
weight proteins up to 400 kd.
B
= Blue Native Gels. New!
Ideal for study of protein-protein interactions and very hydrophobic
proteins, such as membrane proteins, their complexes and super
complexes. (as described by Schagger and von Jagow, Analytical
Biochemistry (1991) 199, 223-231)
Step 3.
Select Gel
Thickness: 75 = 0.75 mm thick
(Great for blotting and they stain quickly, in 15 minutes) 15 = 1.50 mm thick
( More durable and more sample can be loaded) VOTE:
We are considering making mini gels in a 1.0 mm
thickness. Please send us an email if you prefer 1.00 mm thick mini gels.
Service@precastgels.com
Simply type 1 mm Gels in the subject line or leave more
information and we can contact you when they are available. Thank you!
Step 4.
Select your
Running Apparatus: HM = Amersham (Hoefer) Mighty Small, SE250, SE260,
SE280,
Mini VE,Thermo EC120, Fisher Biotech FB VE10-1, or Owl Penguin P8DS
M or
NM = (Universal Cassette)
Novex/Invitrogen XCell I, II, and Surelock, BioRad Mini Protean 3
(BioRad Mini Protean 3 fits & runs one gel at a time),
CBS models MGV-302 and 402, Sigma Aldrich E5889 Vertical Unit,
the ISS/Enproteck/Owl (runs one gel at a time).
BM = Bio-Rad Mini Protean II (but
not Bio-Rad Mini Protean 3, see NM).
Step 5.
Identify the Number of Sample Wells you want:
C10
= 10 sample wells C15
= 15 sample wells
C1
= 1 well. The top of gel is flat and a sample well for standards is not included. The resolving gel is poured 1cm from the top (.5cm from the top for mini gels)
to create a flat top for prep and 2D work
(see 2D gels) . The
C1 designation does not include a stacking gel. If a stacking gel is
desired, you must specify it. C2
= Same as C1 except there is also a sample well (for loading a protein
standard) at one end.
Available on mini
gels with 1.5mm
gel thickness only size Gels. The C2 designation does not include a stacking gel. If a stacking gel is
desired, you must specify it.
C0
= no sample wells (mini gels poured 1.5 cm from top).
Step 6.
Select Plastic Cassette for your
gels: P = Plastic Cassette
Step 7.
Copy your entries from steps 1-6 (in that order) into step 7
and print out.
This is your part
number. For special requests include a note with your order.
Does Your Part Number Look Similar To This Sample? 420D75HMC10P Mini gels are sold 10 per box or 2 per box. You may order gels by phone, fax, or e-mail
or
Order Online.
JULE, INC.
185 Research Drive, #6 Milford, CT 06460
U. S. A. 1-800-648-1772 Service@precastgels.com
,
Order On Line!1-203-878-7411
International and Local Calling Area
Fax: 1-203-878-744 8