SELECTING THE BEST GEL FOR YOUR SAMPLE
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Single Concentration: |
Gradient Concentration: |
| Use low acrylamide concentration gels for larger molecular weight particles. Increasing the acrylamide concentration decreases the gel pore size. Single concentration gels are provided with a 4% stacking gel layered on top of the resolving gel, except for gels with catalog designation C1G or C2G, TBE Gels, and TBE/Urea Gels. A 3% stacking gel is available upon request. Jule, Inc. will pour any single concentration gel. Specify the gel you want (click here)! |
Gradient gels provide sharper bands and they separate a broader range of molecular weight sizes on the same gel. Gradient gels have a lower concentration of acrylamide at the top of the gel and the concentration increases uniformly (linearly) to the bottom of the gel. That is, the gel pore size decreases from the top of the gel to the bottom. Use the gel photographs below to aid in your selection. Prices start at $9.95 each for mini gels. Jule, Inc. will pour any gradient range, however, certain gradient gels have a special low price. Specify the gel you want (click here)! |
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TRIS-GLYCINE GELS TRIS-GLYCINE GELS |
TRICINE |
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| SINGLE CONCENTRATION |
GRADIENT |
GRADIENT |
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| 7.5% | 10% | 12 % | 4-15% | 4%-20% | 8%-16% | 10%-20% |
10%-20% |
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The sample run on each gel above had 13 protein bands, which ranged in size (Daltons) as follows: 212,000 (top of gel), 158,194, 116,351, 97,184, 66,409, 55,561, 42,710, 36,487, 26,625, 20,040, 14,313, 6,517, and 2,340 - 3,400 (Insulin A, B chain) Daltons. Two bands at 66,409 and 26,625 Daltons are double intensity in order to serve as reference points. Notice that in a low acrylamide concentration gel the smaller protein bands can run off of the gel, but larger proteins sizes are well separated. In high acrylamide concentration gels the smaller proteins are better resolved. A gradient gel can resolve large sizes as well as small sizes on the same gel.
- You don't see the Gel that you need? We will pour any single or gradient gel? Simply click here to get a catalog number for the gel you need. All single concentration gels are the same price.
PROTEIN STANDARDS (shown above) are available for purchase:
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Description |
Catalog Number |
Price |
| Broad Range Standards (shown above) |
BRSTD |
$90 for 1,125 uL |
| Prestained Standards (see image) |
PRE-BRSTD |
$120 for 1,050 uL |
* Enough for 150 mini gel lanes, supplied premixed
with loading buffer and reducing agent in one tube (3 month shelf life).
Simply heat prior to use.
The gels above are acrylamide denaturing gels, 0.375 M
Tris-HCl, pH 8.8, 0.1% SDS (available with or without SDS), used with the
Tris-Glycine running buffer (except for
Tricine gels).
Due to the many gel options available to you, refer to CATALOG
NUMBERS FOR PRECAST GELS and PRICE OF MINI AND LARGE
PRECAST GELS.
2-D Gel Electrophoresis
Gel Catalog Numbers
Gel
Prices
