Catalog Numbers for Precast Gels
Since there is a wide selection of gels, please follow the 7 steps below to create your own catalog number.
Call us at 1-800-648-1772 for assistance.
Here is a sample catalog number: 12D15HMC10P
Step 1. Select Acrylamide
Concentration: We will pour almost any single
concentration or gradient concentration
gel.
i.e. 12 = 12% single concentration (Specify any single concentration).
420 = 4%-20% gradient (Pick any gradient you need).
Step 2. Select the Gel
Type: (learn more about buffer
systems)
D = Tris-Glycine Gels with SDS, (Gel buffer is: 0.375M Tris-HCl, pH 8.8,
.1% SDS)
W = Tris-Glycine Gels without SDS (Gel buffer is: 0.375M Tris-HCl, pH 8.8)
N = Tris-Borate-EDTA (TBE), (Gel Buffer is: 0.090M Tris, 0.080M Boric Acid, pH
8.3, 0.0026M EDTA). TBE Gels do not come with a 4% stacking gel.
U = Urea/TBE Gels ( Same as N Gels, with 7 molar Urea added). TBE/Urea
Gels do not come with a 4% stacking gel.
T = Tris-Tricine Gels (ideal for low molecular weight 2 - 60 kilo-Daltons), (Gel
Buffer is: 1.0M Tris-HCl, pH 8.45, 0.1% ) SDS).
Step 3. Select Gel
Thickness:
75 = 0.75 mm thick
15 = 1.50 mm thick
10 = 1.00 mm thick (available for Ettan Dalt large
"EL", "HL", and "BL" style cassettes.
only)
Step 4. Select your
Running Apparatus:
MINI GELS:
HM = Amersham (Hoefer) Mighty Small, SE250, SE260,
SE280,
Thermo EC120, or Owl Penguin P8DS
NM = (Universal Cassette) Novex/Invitrogen XCell I, II, and Surelock, BioRad Mini Protean 3
CBS models MGV-302 and 402, Sigma Aldrich E5889 Vertical Unit.
BM = Bio-Rad Mini Protean II (but not Bio-Rad Mini Protean 3, see NM)
SM = Owl/ISS/Enprotech Min Apparatus or Hoefer SE200 use glass cassettes. These
glass mini cassettes also fit into the same apparatus as "HM" and "BM".
These mini cassettes 8cm tall x 10cm wide are made with low fluorescence glass.
LARGE SIZE GELS
HL = Amersham (Hoefer) SE400, SE600, SE650, SE700
(Standard gel width is 14 cm. A
16 cm wide gel can be obtained by requesting 1.0 cm wide spacers).
BL = Bio-Rad Protean II and IIxi, Owl P10DS-1,-2,-3 (Owl requires sponge tips,
ST-10). (Standard Gel width is 16 cm. An 18 cm
wide gel can be obtained by requesting 1.0 cm wide spacers. Cassettes
dimensions: front plate 16cm tall x 20 cm wide and back plate 18.3 cm tall by 20
cm wide.)
BXL = Bio-Rad Protean IIxi (20 cm), and XL (20 cm)
(An 18 cm wide gel is obtained using 1.0 cm wide spacers. Cassette dimensions:
Front plate 20cm tall x 20 cm wide, back plate 22.3 cm tall x 20 cm wide.)
Currently available in low fluorescence glass only.
EL = Ettan Dalt Six and Twelve Large Vertical System, the
BioRad Protean Plus. These are made with low fluorescence glass.
Gel width is 25.5 cm wide. Cassette dimensions: 21.7
cm tall x 27.6 cm wide. Please remember to specify in words along with
your catalog number whether or not you would like Bind Silane, Repel Silane, and
2 fluorescent markers and whether to apply the Bind Silane to the Short or tall
plates. Or specify No Bind, No Repel and No Markers, for example.
Step 5.
Identify the Number of Sample Wells you want:
C0 = no sample wells (mini gels poured 1.5 cm from top, large gels poured 3.5 cm
from top)
C10 = 10 wells
C15 = 15 wells
C1 = 1 well. the resolving gel is poured 1cm from top (.5cm from the top for mini gels) or prep and 2D work
(see 2D gels) The
C1 designation does not include a stacking gel. If a stacking gel is
desired, you must specify it.
C2 = same as C1 except there is also a reference well at one end. Only
available on "HM", "BM","NM","HL" and "BL" size Gels.
The C2 designation does not include a stacking gel. If a stacking gel is
desired, you must specify it.
Step 6. Select a Plastic or Glass Cassette for your
gels:
P = Plastic Cassette (available for 'HM', 'BM', 'NM' styles and running one gel
in "SM" style)
G = Glass Cassette (all large gels use glass cassettes. Use glass to run 2 gels
in "SM")
GLF=low fluorescence glass (available for "EL", "HL", "BL" and "BXL"). "EL" are made with Low fluorescence glass. "HL" and "BL" can be made with regular glass "G" or low fluorescence glass "GLF". Our Mini Gels "SM" style are made with low fluorescence glass also.
Step 7.
Copy your entries
from steps 1-6 (in that order) into the field above
This is your part
number. For special requests include a note with your order. Ettan
Dalt users must specify Options in words along with catalog number. Any
additional instructions must be specified in words .
Does Your Part Number Look Similar To This Sample? 420D75HMC10P
Mini gels are sold 10 per box or 2 per box (glass
cassettes 8 per box or 2 per box),
Large size gels are sold 6 per box or 2 per box.
You can order more than 2, 6, 8 or 10 gels quantities.
You may order gels by phone, fax, or e-mail.
Gel Selection Guide (see pictures of Snap-A-Gels)
2-D Gel Electrophoresis
Gel
Prices
Order Online
Tricine Gels for low molecular weight proteins