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Jule Precast Gels Here is a sampling of some recently published papers written by users of Jule, Inc products! Partial list below! Click on links to see papers: http://scholar.google.com/scholar?hl=en&scoring=r&q=jule+precast+gels&as_ylo=2004 Recent papers.
... Ten-microliter aliquots of the denatured samples were then loaded onto a 16% Non-Gradient Tris-Tricine gel (Jule Inc., Milford, Connecticut). ...
... Fisher (Pittsburgh, PA). Gels for Ettan Dalt Six System GE Healthcare were purchased from Jule, Inc. (Milford, CT)
... Equal quantities of proteins were electrophoresed on 420% gradient gels (Jule Inc., Milford, CT, USA);
... Immediately prior to Western blotting (described below), 2Χ loading buffer (Jule, Inc., Milford, CT) was added to the samples.
... pellet was rehomogenized in 1 ml buffer A and layered on top of a linear 1045% sucrose gradient (11 ml, prepared using J17 Model gradient maker from Jule Inc ...
... The equilibrated strips were loaded onto precast 2-DE gels with exponential gradients of 10 to 20% (wt/vol) acrylamide (Jule, Inc., Milford, CT). ...
... Samples were also analyzed on 16 x 16 x 1.5-mm, 8% to 16% gradient gels (Jule Inc., Milford, CT) and stained with flamingo pink fluorescent protein stain...
... Strips were then applied to a precast 8% to 16% SDS-PAGE gel (Jule Inc., Milford, CT) for electrophoresis at a constant 20 W/gel at 25°C in an Ettan DALT 12 ...
... gels and buffers. Pre-cast native polyacrylamide gradient gels (4-20%) were purchased from Jule Inc. (Milford, CT).
... The 2D Sample Prep for Membrane Proteins Kit and the 1020% gradient TrisGlycine gels were from Jule Inc (Milford, CT)
... equilibration buffer. The second dimension was carried out using 12% PAGE tris-glycine gels (Jule, Inc, Milford, CT).
... Cl, 4% SDS, 20% v/v glycerol, 0.2 M dithiothreitol, and 0.02% bromophenol blue, pH 6.8), applied to 12.5% precast acrylamide minigels (Jule, Inc., Milford, CT ...
... loaded onto an Ettan DALT-12 electrophoresis unit (GE Healthcare), and the proteins were separated on a 10-15% SDS-polyacrylamide gel (Jule Inc., Milford, CT ...
KL Rosewell, TE Curry Jr - Methods in molecular biology (Clifton, NJ), 2009 Springer ... 117 Page 4. 11. Snap-A-GelsTM mini cassette, 1.5 mm (Jule, Inc.) 12. Gel Loading Buffer II (Ambion) 13. Kodak Biomax XAR film (Thermo Fisher). 2.3. ...
... 46 mg/ml SDS, 0.2 mg/ml bromophenol blue, 20 mg/ml DTT), boiled for 5 min, loaded onto a 420% gradient TrisSDS gel (Jule Inc.), electrophoresed at 200 V ...
MB Lucitt, TS Price, A Pizarro, W Wu, AK - Molecular & Cellular Proteomics, 2008 ASBMB IPG strips were then placed on top of an 816% precast polyacrylamide gel (Jule, Inc., Milford, CT).
... Western blotting was performed with Trisglycine precast 10% SDS gels (16 Χ 18 cm) with 10 lanes (Jule, Inc., Milford, Conn.), ...
.. N-α-Syn IP samples were fractionated by large format 16% Tricine SDS-PAGE (Jule Inc., Milford, CT; BIORAD Laboratories, Inc, Los Angeles, CA) at constant ...
RT Netea-Maier, SW Hunsucker, BM - Cancer Research, 2008 AACR ... Gel electrophoresis was performed on precast 8% to 16% acrylamide gradient gels (Jule, Inc.) as previously described (8).
L Xu, X Pei, K Berzins, A Chaudhuri - Experimental parasitology, 2007 Elsevier ... by specific antibodies. MRSPs were analyzed on a 327% precast gradient gel (JULE Inc., Research Drive, Milford, CT).
... Linear 12 ml 1025% sucrose density gradients in 1ΧHMDEK buffer plus protease inhibitors were generated by using the Jule Gradient Former (Jule, Inc. ...
... of sMBL, hMBL, MN and TP were mixed with equal volumes (7.5 ΅l) of sample buffer prior to loading into wells of Snap-A-Gel precast gels (Jule Inc., USA). ...
JJ Zoeller, RV Iozzo - Proteome Science, 2008 - proteomesci.com ... containing 2.5% iodoacetamide. IPG strips were then directly applied to 818% SDS Tris-Glycine gels (Jule Inc. Biotechnologies), ...
... Pre-cast 815% gradient SDS-PAGE (2,400 2,000 1 mm; Jule Inc.); Fixing solution (30% (vol/vol) methanol and 7.5% (vol/vol) acetic acid); ...
M Ehrich, S Zoll, S Sur, D van den Boom - Nucleic Acids Research, 2007 - Oxford Univ Press ... Ten microliters of the bisulfite-treated DNA was loaded along with 5 ΅l of loading dye on a 48% TBE PAGE pre-cast gel (Jule Inc., Milford, CT). ...
LN Waller, MJ Stump, KF Fox, WM Harley, A - Journal of bacteriology, 2005 - Am Soc Microbiol ... 5% ί-mercaptoethanol, 0.02% bromophenol blue [Fisher Scientific]), loaded onto each lane of 4 to 15% gradient polyacrylamide gels (Jule, Inc., Milliford, CT ...
RA Garrick, BR Woodin, JY Wilson, BL - Aquatic Toxicology, 2006 Elsevier ... of standards of CYP1A from scup (Stenotomus chrysops) were resolved on a 420% acrylamide gradient gel in TRISglycine with SDS (Jule Inc., Milford, CT). ...
N Tannu, DC Mash, SE Hemby - Molecular psychiatry, 2006 - nature.com ... on the basis of their molecular weight on a pre-cast 815% gradient SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (2400 2000 1 mm, Jule Inc., Milford, CT ...
PC Burcham, FR Fontaine, LM Kaminskas, DR - Molecular pharmacology, 2004 ASPET ... at 4°C. After protein estimation (Pierce BCA Kit), 50 ΅g of protein was resolved overnight at 4 V/cm on either a 4 to 20% gradient acrylamide gel (Jule Inc. ...
MK Gupta, V Subramanian, JS Yadav - J. Proteome Res, 2009 - pubs.acs.org ... subsequent effort to improve the 2DE-resolution of the secreted proteins, a larger IPG strip (24 cm) and a precast 4−20% gradient gel (Jule, Inc., Milford, CT ...
RA Garrick, BR Woodin, JJ Stegeman - In Vitro Cellular & Developmental Biology-Animal, 2005 Springer ... of CYP1A from scup (Stenotomus chrysops) were resolved on a 4-20% acrylamide gradient gel in TRIS-glycine with sodium dodecyl sulfate (Jule Inc., Milford, CT). ...
... To quantitate the apoE, 200 μg/well of denatured protein samples were separated on 4%20% gradient SDS-PAGE (Jule Inc, Milford, CT), ...
MA Shifman, Y Li, CM Colangelo, KL Stone, - J. Proteome Res, 2007 - pubs.acs.org ... with CyDye DIGE fluor labeling reagents, GE Healthcare Immobiline DryStrip Gels, the Ettan IPGphor IEF System, and pre-poured polyacrylamide gels from Jule Inc ...
JP Folsom, JF Frank - Journal of Food Protection&# 174;, 2007 - ingentaconnect.com ... instructions. IPG strips were loaded onto 1 mM Tris-glycine gra- dient (8 to 15%) gels (Jule, Inc., Milford, Conn.).
... HS-acclimated fish and 2−5 μg from oil-exposed fish) were electrophoretically resolved on 5−20% gradient gels (Precast Snap-A-Gel, Jule Inc.),
... CYP1A from scup (Stenotomus chrysops) were resolved on a 4-20 % acrylamide gradient gel in TRIS-glycine with SDS (Jule,Inc, Milford, CT)...
... et al. 1988) were dissolved in 1Χ Laemmli SDS sample buffer and run on 4%-12% gradient gel (Jule, Inc.)...
AU Uebing-Czipura, HD Dawson, G Scherba - Journal of neuroscience methods, 2008 Elsevier ... TPG) for each sample, the amplicons were separated during vertical electrophoresis in a non-denaturing 12% polyacrylamide gel (Jule, Inc., Milford, CT) ...
W Richter, M Conti - Journal of Biological Chemistry, 2004 ASBMB ... and 5 mM -mercaptoethanol were prepared in 14 x 89-mm centrifugation tubes (Beckman Instruments, Fullerton, CA) using a gradient former (Jule Inc., Milford, CT ...
Z Tian, AS Greene, JL Pietrusz, IR Matus, M - Genome research, 2008 - 171.66.122.45
... buffer containing 0.5% DTT, then 15
min in the same buffer with 4.5% (w/v) iodoacetamide, and then loaded onto 12.5%
vertical SDS-PAGE
slab gels (Jule Inc.). ... |
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