Jule Precast Gels

Here is a sampling of some recently published papers written by users of Jule, Inc products!

Partial list below! Click on links to see papers: 

 

bullet Liposomes targeted by fusion phage proteins
PK Jayanna, VP Torchilin, VA Petrenko - Nanomedicine: Nanotechnology, Biology, and …, 2009 – Elsevier

... Ten-microliter aliquots of the denatured samples were then loaded onto a 16% Non-Gradient Tris-Tricine gel (Jule Inc., Milford, Connecticut). ... 

bullet HIV-1 infected monocyte-derived macrophages affect the human brain microvascular …
M Ricardo-Dukelow, I Kadiu,
W Rozek, J … - Journal of neuroimmunology, 2007 – Elsevier

... Fisher (Pittsburgh, PA). Gels for Ettan Dalt Six System GE Healthcare were purchased from Jule, Inc. (Milford, CT)…   

bullet Adenoviral transfer of vasoactive intestinal peptide (VIP) gene inhibits rat aortic and …
RC St. Hilaire, PJ Kadowitz, JR Jeter - Peptides, 2009 – Elsevier

 ... Equal quantities of proteins were electrophoresed on 4–20% gradient gels (Jule Inc., Milford, CT, USA); … 

bullet HLA-G is found in lipid rafts and can act as a signaling molecule
M Comiskey, KE Domino, CM Warner - Human immunology, 2007 – Elsevier

... Immediately prior to Western blotting (described below), 2Χ loading buffer (Jule, Inc., Milford, CT) was added to the samples. … 

bullet Plasma membrane cyclic nucleotide phosphodiesterase 3B (PDE3B) is associated with …
R Nilsson, F Ahmad, K Swδrd, U Andersson, … - Cellular signaling, 2006 – Elsevier

 ... pellet was rehomogenized in 1 ml buffer A and layered on top of a linear 10–45% sucrose gradient (11 ml, prepared using J17 Model gradient maker from Jule Inc ...  

bullet Comparison of the proteosomes and antigenicities of secreted and cellular proteins …
D Cho, MT Collins - Clinical and Vaccine Immunology, 2006 - Am Soc Microbiol

 ... The equilibrated strips were loaded onto precast 2-DE gels with exponential gradients of 10 to 20% (wt/vol) acrylamide (Jule, Inc., Milford, CT). ...  

bullet Mitochondrial proteomics of the retinal pigment epithelium at progressive stages of age- …
CL Nordgaard, PP Karunadharma, X Feng, TW … - Investigative Ophthalmology & Visual Science, 2008 – ARVO

 ... Samples were also analyzed on 16 x 16 x 1.5-mm, 8% to 16% gradient gels (Jule Inc., Milford, CT) and stained with flamingo pink fluorescent protein stain...   

bullet Gelsolin is Depleted in Post-Shock Mesenteric Lymph
JR Jordan, EE Moore, SS Damle, P Eckels, … - Journal of Surgical Research, 2007 – Elsevier

... Strips were then applied to a precast 8% to 16% SDS-PAGE gel (Jule Inc., Milford, CT) for electrophoresis at a constant 20 W/gel at 25°C in an Ettan DALT 12 ...  

bullet RPE65 is an iron (II)-dependent isomerohydrolase in the retinoid visual cycle
G Moiseyev, Y Takahashi, Y Chen, S … - Journal of Biological Chemistry, 2006 – ASBMB

 ... gels and buffers. Pre-cast native polyacrylamide gradient gels (4-20%) were purchased from Jule Inc. (Milford, CT). …  

bullet HIV-1 transforms the monocyte plasma membrane proteome
I Kadiu, T Wang, JD Schlautman, L … - Cellular Immunology, 2009 – Elsevier

... The 2D Sample Prep for Membrane Proteins Kit and the 10–20% gradient Tris–Glycine gels were from Jule Inc (Milford, CT) … 

bullet A Comparison of Protein Extraction Methods Suitable for Gel-Based Proteomic Studies of …
M Cilia, T Fish, X Yang, M Mclaughlin, TW … - 2009 - pubmedcentral.nih.gov

... equilibration buffer. The second dimension was carried out using 12% PAGE tris-glycine gels (Jule, Inc, Milford, CT). … 

bullet Redox-reactive membrane vesicles produced by Shewanella
Y Gorby, J Mclean, A Korenevsky, K Rosso, … - Geobiology, 2008 - nanobio.usc.edu

... Cl, 4% SDS, 20% v/v glycerol, 0.2 M dithiothreitol, and 0.02% bromophenol blue, pH 6.8), applied to 12.5% precast acrylamide minigels (Jule, Inc., Milford, CT ...

bullet The mammalian target of rapamycin (mTOR) pathway regulates mitochondrial oxygen …
SM Schieke, D Phillips, JP McCoy Jr, AM … - Journal of Biological Chemistry, 2006 – ASBMB

... loaded onto an Ettan DALT-12 electrophoresis unit (GE Healthcare), and the proteins were separated on a 10-15% SDS-polyacrylamide gel (Jule Inc., Milford, CT ...

bullet Detection of Ovarian Matrix Metalloproteinase mRNAs by In Situ Hybridization.

KL Rosewell, TE Curry Jr - Methods in molecular biology (Clifton, NJ), 2009 – Springer

... 117 Page 4. 11. Snap-A-GelsTM mini cassette, 1.5 mm (Jule, Inc.) 12. Gel Loading Buffer II (Ambion) 13. Kodak Biomax XAR film (Thermo Fisher). 2.3. ...  

bullet Gene targeting reveals the role of Oc90 as the essential organizer of the otoconial organic …
X Zhao, H Yang, EN Yamoah, YW Lundberg - Developmental biology, 2007 – Elsevier

... 46 mg/ml SDS, 0.2 mg/ml bromophenol blue, 20 mg/ml DTT), boiled for 5 min, loaded onto a 4–20% gradient Tris–SDS gel (Jule Inc.), electrophoresed at 200 V ...

bullet Analysis of the zebrafish proteome during embryonic development

MB Lucitt, TS Price, A Pizarro, W Wu, AK … - Molecular & Cellular Proteomics, 2008 – ASBMB

…IPG strips were then placed on top of an 8–16% precast polyacrylamide gel (Jule, Inc., Milford, CT). …

bullet Nuclear factor kappa Β in the dorsal raphe of macaques: an anatomical link for steroids, …
CL Bethea, AP Reddy, LJ Smith - Journal of Psychiatry & Neuroscience, 2006 - pubmedcentral.nih.gov

... Western blotting was performed with Tris–glycine precast 10% SDS gels (16 Χ 18 cm) with 10 lanes (Jule, Inc., Milford, Conn.), ...  

bullet Nitrated α–Synuclein Immunity Accelerates Degeneration of Nigral Dopaminergic Neurons
EJ Benner, R Banerjee, AD Reynolds, S … - PLoS
ONE, 2008 - pubmedcentral.nih.gov

.. N-α-Syn IP samples were fractionated by large format 16% Tricine SDS-PAGE (Jule Inc., Milford, CT; BIORAD Laboratories, Inc, Los Angeles, CA) at constant ...   

bullet Discovery and validation of protein abundance differences between follicular thyroid …

RT Netea-Maier, SW Hunsucker, BM … - Cancer Research, 2008 – AACR

... Gel electrophoresis was performed on precast 8% to 16% acrylamide gradient gels (Jule, Inc.) as previously described (8). …  

bullet Plasmodium yoelii: experimental evidences for the conserved epitopes between mouse and …

L Xu, X Pei, K Berzins, A Chaudhuri - Experimental parasitology, 2007 – Elsevier

... by specific antibodies. MRSPs were analyzed on a 3–27% precast gradient gel (JULE Inc., Research Drive, Milford, CT). …  

bullet Intraflagellar Transport (IFT) Protein IFT25 Is a Phosphoprotein Component of IFT Complex …
Z Wang, ZC Fan, SM Williamson, H Qin - PLoS
ONE, 2009 - pubmedcentral.nih.gov

... Linear 12 ml 10–25% sucrose density gradients in 1ΧHMDEK buffer plus protease inhibitors were generated by using the Jule Gradient Former (Jule, Inc. ...  

bullet The interaction of mannose binding lectin (MBL) with mannose containing glycopeptides and …
PI Sealy, B Garner, E Swiatlo, SW Chapman, … - Medical Mycology, 2008 - informahealthcare.com

... of sMBL, hMBL, MN and TP were mixed with equal volumes (7.5 ΅l) of sample buffer prior to loading into wells of Snap-A-Gel™ precast gels (Jule Inc., USA). ...  

bullet Proteomic profiling of endorepellin angiostatic activity on human endothelial cells

JJ Zoeller, RV Iozzo - Proteome Science, 2008 - proteomesci.com

... containing 2.5% iodoacetamide. IPG strips were then directly applied to 8–18% SDS Tris-Glycine gels (Jule Inc. Biotechnologies), ...  

bullet Two-dimensional fluorescence difference gel electrophoresis for comparative proteomics …
NS Tannu,
SE Hemby - Nature protocols, 2006 - nature.com

... Pre-cast 8–15% gradient SDS-PAGE (2,400 2,000 1 mm; Jule Inc.); Fixing solution (30% (vol/vol) methanol and 7.5% (vol/vol) acetic acid); ...  

bullet A new method for accurate assessment of DNA quality after bisulfite treatment

M Ehrich, S Zoll, S Sur, D van den Boom - Nucleic Acids Research, 2007 - Oxford Univ Press

... Ten microliters of the bisulfite-treated DNA was loaded along with 5 ΅l of loading dye on a 4–8% TBE PAGE pre-cast gel (Jule Inc., Milford, CT). ...

bullet Identification of a second collagen-like glycoprotein produced by Bacillus anthracis and …

LN Waller, MJ Stump, KF Fox, WM Harley, A … - Journal of bacteriology, 2005 - Am Soc Microbiol

... 5% ί-mercaptoethanol, 0.02% bromophenol blue [Fisher Scientific]), loaded onto each lane of 4 to 15% gradient polyacrylamide gels (Jule, Inc., Milliford, CT ...  

bullet Cytochrome P4501A is induced in endothelial cell lines from the kidney and lung of the …

RA Garrick, BR Woodin, JY Wilson, BL … - Aquatic Toxicology, 2006 – Elsevier

 ... of standards of CYP1A from scup (Stenotomus chrysops) were resolved on a 4–20% acrylamide gradient gel in TRIS–glycine with SDS (Jule Inc., Milford, CT). ...  

bullet Cytosolic proteomic alterations in the nucleus accumbens of cocaine overdose victims

N Tannu, DC Mash, SE Hemby - Molecular psychiatry, 2006 - nature.com

... on the basis of their molecular weight on a pre-cast 8–15% gradient SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (2400 2000 1 mm, Jule Inc., Milford, CT ...

bullet Protein adduct-trapping by hydrazinophthalazine drugs: mechanisms of cytoprotection …

PC Burcham, FR Fontaine, LM Kaminskas, DR … - Molecular pharmacology, 2004 – ASPET

... at 4°C. After protein estimation (Pierce BCA Kit), 50 ΅g of protein was resolved overnight at 4 V/cm on either a 4 to 20% gradient acrylamide gel (Jule Inc. ...  

bullet Immunoproteomic Identification of Secretory and Subcellular Protein Antigens and …

MK Gupta, V Subramanian, JS Yadav - J. Proteome Res, 2009 - pubs.acs.org

... subsequent effort to improve the 2DE-resolution of the secreted proteins, a larger IPG strip (24 cm) and a precast 4−20% gradient gel (Jule, Inc., Milford, CT ...  

bullet Cytochrome P4501A induced differentially in endothelial cells cultured from different organs …

RA Garrick, BR Woodin, JJ Stegeman - In Vitro Cellular & Developmental Biology-Animal, 2005 – Springer

... of CYP1A from scup (Stenotomus chrysops) were resolved on a 4-20% acrylamide gradient gel in TRIS-glycine with sodium dodecyl sulfate (Jule Inc., Milford, CT). ...

bullet Effect of human apolipoprotein E genotype on the pathogenesis of experimental ocular HSV …
PS Bhattacharjee, DM Neumann, TP Foster, S … - Experimental eye research, 2008 – Elsevier

... To quantitate the apoE, 200 μg/well of denatured protein samples were separated on 4%–20% gradient SDS-PAGE (Jule Inc, Milford, CT), ...

bullet YPED: a web-accessible database system for protein expression analysis

MA Shifman, Y Li, CM Colangelo, KL Stone, … - J. Proteome Res, 2007 - pubs.acs.org

... with CyDye DIGE fluor labeling reagents, GE Healthcare Immobiline DryStrip Gels, the Ettan IPGphor IEF System, and pre-poured polyacrylamide gels from Jule Inc ...

bullet Proteomic analysis of a hypochlorous acidtolerant Listeria monocytogenes cultural variant …

JP Folsom, JF Frank - Journal of Food Protection&# 174;, 2007 - ingentaconnect.com

... instructions. IPG strips were loaded onto 1 mM Tris-glycine gra- dient (8 to 15%) gels (Jule, Inc., Milford, Conn.). …

bullet Humic substances and crude oil induce cytochrome P450 1A expression in the Amazonian …
AYO Matsuo, BR Woodin, CM Reddy, AL Val, … - Environ. Sci. Technol, 2006 - pubs.acs.org

... HS-acclimated fish and 2−5 μg from oil-exposed fish) were electrophoretically resolved on 5−20% gradient gels (Precast Snap-A-Gel, Jule Inc.), … 

bullet Cytochrome P450 induced differentially in endothelial cells cultured from different organs of …
RA Garrick, BR Woodin, JJ Stegeman - 2004 - darchive.mblwhoilibrary.org

... CYP1A from scup (Stenotomus chrysops) were resolved on a 4-20 % acrylamide gradient gel in TRIS-glycine with SDS (Jule,Inc, Milford, CT)...  

bullet WUSCHEL is a primary target for transcriptional regulation by SPLAYED in dynamic control of …
CS Kwon, C Chen, D Wagner - Genes & development, 2005 - genesdev.cshlp.org

... et al. 1988) were dissolved in 1Χ Laemmli SDS sample buffer and run on 4%-12% gradient gel (Jule, Inc.)...  

bullet Immortalization and characterization of lineage-restricted neuronal progenitor cells derived …

AU Uebing-Czipura, HD Dawson, G Scherba - Journal of neuroscience methods, 2008 – Elsevier

... TPG) for each sample, the amplicons were separated during vertical electrophoresis in a non-denaturing 12% polyacrylamide gel (Jule, Inc., Milford, CT) ...  

bullet The oligomerization state determines regulatory properties and inhibitor sensitivity of type …

W Richter, M Conti - Journal of Biological Chemistry, 2004 – ASBMB

... and 5 mM -mercaptoethanol were prepared in 14 x 89-mm centrifugation tubes (Beckman Instruments, Fullerton, CA) using a gradient former (Jule Inc., Milford, CT ...  

bullet MicroRNA–target pairs in the rat kidney identified by microRNA microarray, proteomic, and …

Z Tian, AS Greene, JL Pietrusz, IR Matus, M … - Genome research, 2008 - 171.66.122.45

 ... buffer containing 0.5% DTT, then 15 min in the same buffer with 4.5% (w/v) iodoacetamide, and then loaded onto 12.5% vertical SDS-PAGE slab gels (Jule Inc.). ...
 

 

To Choose the Gels you need follow these 4 Steps:

1) Choose Acrylamide Gel Concentration See images of actual proteins run on gels.
2) Choose Gel Part Number Develop a part number by choosing the options available such as: gel concentration, gel buffer, thickness, cassette size, sample wells, etc.
3) Gel Prices See prices of all gel types and Sizes.
4) Place an Order How to order information: Fax number, Phone number or online ordering.
 
 
 

DOWNLOADS

Precast Gel Quick Guide (.pdf, 670kb) Information on power settings, sample well volumes, etc.

Gel Manual and Trouble Shooting Guide (.pdf, 34kb)

Running Buffers, Sample Buffers, Protein Standards, (.pdf, 34kb)

Gradient Former Manual

 

Other:

Accessories

Product Literature

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Papers Published By Jule Gel Users

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Revised: March 03, 2010