Jule precast polyacrylamide gels selection guide.

Single Concentration:

Gradient Concentration:

Use low acrylamide concentration gels for larger molecular weight particles. Increasing the acrylamide concentration decreases the gel pore size.

Single concentration gels are provided with a 4% stacking gel layered on top of the resolving gel, except for gels with catalog designation one or two sample wells, TBE Gels, and TBE/Urea Gels. A 3% stacking gel is available upon request. Jule, Inc. will pour any single concentration gel.

Gradient gels provide sharper bands and they separate a broader range of molecular weight sizes on the same gel. Gradient gels have a lower concentration of acrylamide at the top of the gel and the concentration increases uniformly (linearly) to the bottom of the gel. That is, the gel pore size decreases from the top of the gel to the bottom. Use the gel photographs below to aid in your selection.

Prices start at $8.95 each for mini gels. Jule, Inc. will pour any gradient range.

TRIS-GLYCINE GELS¹								Tricine Gels² (About Tricine Gels)	TBE Gels³
SINGLE CONCENTRATION			GRADIENT					GRADIENT	Single
7.5%	10%	12 %		4-15%	4-20%	8-16%	10-20%	10-20%	20% with10%Stack

The sample run on each gel above had 13 protein bands, which ranged in size (Daltons) as follows: 212,000 (top of gel), 158,194, 116,351, 97,184, 66,409, 55,561, 42,710, 36,487, 26,625, 20,040, 14,313, 6,517, and 2,340 - 3,400 (Insulin A, B chain) Daltons. Two bands at 66,409 and 26,625 Daltons are double intensity in order to serve as reference points. Notice that in a low acrylamide concentration gel the smaller protein bands can run off of the gel, but larger proteins sizes are well separated. In high acrylamide concentration gels the smaller proteins are better resolved. A gradient gel can resolve large sizes as well as small sizes on the same gel.

1) Tris-HCl Gels also called Tris Glycine Gels	2) Tris-Tricine Gels:	3) Tris Borate EDTA (TBE) Gels
Gel buffer is 0.375 M Tris-HCl, pH 8.8, 0.1% SDS (available with or without SDS), used with the Tris-Glycine running buffer.	Gel buffer is 1.0 M Tris-HCl, pH 8.45, 0.1 % SDS. Use with Tris-Tricine Running Buffer.	The Gel buffer is 0.090 Molar Tris, 0.080 Molar Boric Acid, pH 8.3, 0.0026M EDTA. Use with TBE running buffer. Bands on above gel are 10 to 300 base pairs.